基于RGB颜色空间的早稻氮素营养监测研究

针对双季稻区水稻过量施肥带来环境污染和成本提高问题,设计不同品种氮肥梯度大田试验,应用数码相机获取早稻冠层数字图像,研究不同色彩参数及早稻氮素营养指标的时空变化特征,以期确立双季早稻氮素营养预测模型。结果表明:不同品种同一氮肥处理下图像色彩参数差异不大;拔节期数字图像参数对氮素营养指标敏感;模型构建结果显示,图像参数INT与水稻氮素营养指标构建的模型决定系数(R2)最大,模型预测效果最佳,R2分别为0.895 7和0.924 7;进一步采用多元回归分析和BP神经网络分析法进行预测,预测效果均较好。对预测结果进行检验,发现品种对于模型的构建影响不大,以BP神经网络分析法构建的叶片氮浓度(LNC)模型和以INT为敏感色彩参数构建的叶片氮积累量(LNA)回归模型效果最优,而多元回归分析方法则效果不佳。早稻冠层RGB颜色空间敏感参数与氮素营养指标间相关性较好,可以实现氮素营养的无损监测诊断。     

有益生防菌包衣处理不同油菜品种种子对菌核病发病率及产量的影响研究

油菜菌核病是油菜上的重要病害之一，在四川局部地区油菜根肿病时有发生，严重威胁到油菜安全生产。以油菜菌核病和根肿病为防治对象，与华中农业大学合作，利用其筛选的有益生防菌，选择双低和高芥酸油菜品种国豪油8号和绵油309，对其种子进行包衣处理，分别在无和有根肿病史的水旱轮作地，开展田间试验。试验结果表明：有益生防菌包衣处理的菌核病发病率均低于没有包衣处理；包衣处理的不同品种产量均高于没有包衣处理，但品种间有差异；包衣处理过的高芥酸油菜品种绵油309的产量高于包衣处理过的双低油菜品种国豪油8号；在有根肿病史试验田种植的菌核病发病率低于无根肿病史试验田，产量高于无根肿病史试验田。包衣处理对油菜根肿病的防治效果不显著。     

兴宁市优质稻高产品种种植示范及其配套技术

近几年来兴宁市积极推广优质稻的生产,种植面积逐年增加。结合兴宁市优质稻栽培实践,总结了目前的示范推广情况,最后提出了配套的技术要点。     

施用锌肥和遮阴互作对花生生长发育、抗病性及产量的影响

采用大田裂区试验，研究了施用锌肥和不同遮阴程度互作对花生生长发育、抗病性及产量的影响。结果表明，与不施锌肥相比，施用锌肥能提高花生不同部位锌含量、增加叶片SPAD值，提高花生叶片中可溶性糖、蛋白质和生长素含量，减少花生病害的发生，平均降低7.1个百分点，花生产量平均增加19.4%。相同施锌水平下，随着遮阴程度的增加，花生不同部位锌的含量和不同生育期叶片SPAD值以及花生叶片中可溶性糖、蛋白质和生长素含量呈增加趋势，花生的发病率比不遮阴对照增加4.8、10.2个百分点，花生产量平均降低16.5%、10.0%。在30%、70%的遮阴条件下，施用锌肥的花生产量比不施锌的分别提高21.1%、25.0%。本试验条件下，施用七水硫酸锌30 kg/hm2，使花生具有较强的抗低温寡照能力及抗病性能，增产显著，可在花生产区推广应用。     

不同水肥浓度对涌泉根灌枣树叶面积和产量的影响

以陕西榆林山地矮化密植梨枣树为研究对象,分析了不同水肥浓度对涌泉根灌枣树生育期内叶面积指数、果实发育和产量的影响。结果表明,不同水肥处理叶面积指数在枣树果实膨大期达到高峰,且稳定持续至果实成熟期末期,随后叶面积指数开始下降。叶面积指数随着萌芽展叶期灌水量的增加而增加,所有水肥处理下,枣数叶面积指数最大的是高水中肥处理(W1N2)；所有处理中,高水、低水处理与低肥相组合,果实体积最大,中灌水处理为不施肥时果实的体积最大。单位枝长的果实数目最高的是中水中肥处理(W2N2),最低的是低水处理(W3),在一定范围内,适当施肥能促进枣果单位枝长的果实数目的增加,但肥液浓度过大,反而会抑制其增加。在一定条件下,所有处理中产量最高的是中水中肥处理(W2N2),产量超过2000kg/亩,最低的是低水高肥处理(W3N1),产量不到1000kg/亩。因此可以选择中水中肥处理(W2N2),能获得最高的产量。     

Effects of taurine-zinc on learning and memory abilities of vascular dementia mice

AIM:To observe the effects of taurine-zinc (TZC) on the learning and memory abilities of vascular dementia (VD) mice and to investigate the related mechanism. METHODS:The mice were randomly divided into model group, sham group, and TZC at 50 mg/kg, 100 mg/kg and 200 mg/kg groups. The mice in drug groups were given TZC by gavage at 10 mL/kg once daily. The mice in sham group and model group were given equal volume of distilled water. VD mice were established by intercepting both common carotid arteries and bleeding at caudal vein after 14 d of gavage. The levels of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) were detected by ELISA. The levels of inducible nitric oxide synthase (iNOS) and nitric oxide (NO) were measured via spectrophotometer. Step-down test and Morris water maze test were used to examine the abilities of learning and memory in the mice. RESULTS:TZC at 50 mg/kg, 100 mg/kg and 200 mg/kg reduced the levels of TNF-α, IL-1β, iNOS and NO in the brain tissues. In the water maze test, TZC at 100 mg/kg and 200 mg/kg significantly decreased the error times and latency compared with model group. In the step-down test, the escape latency was prolonged and error times were lowered significantly by treatment with TZC at 50 mg/kg, 100 mg/kg and 200 mg/kg as compared with model group. CONCLUSION:TZC improves the abilities of learning and memory, which might be related to the reduction of TNF-α, IL-1β, iNOS and NO levels in VD mice.     

果蔬型甜玉米新品种京科甜191的选育

京科甜191是以超甜玉米自交系T68为母本,自交系T6302为父本,通过杂交选育而成的果蔬型甜玉米品种。果穗筒型,穗长19.0 cm,穗粗5.3 cm,穗行数16~18行,行粒数36~38粒,粒行整齐。籽粒亮黄色,外观漂亮。平均每667 m2鲜果穗产量900 kg左右,出籽率约67.4%。鲜籽粒含粗蛋白2.98%,粗脂肪1.37%,总糖6.7%,口感甜脆清爽,皮薄无渣。播种至采收平均85.4 d(天),适宜京津冀及类似生态区种植。     

Assessing vulnerability of New Zealand lakes to loss of conservation value from invasive fish impacts

1. Predictions of invasion risk for seven non‐indigenous fish species, ecological impact scores for individual species, and lake conservation rankings were linked to develop Invasion Risk Impact (IRI) and Lake Vulnerability (LV) indices that help identify New Zealand lakes most at risk of loss of conservation value from potential multi‐species invasions.
2. Species‐specific IRI scores (the product of predicted invasion risk and species impact) highlighted Eurasian perch (Perca fluviatilis) and the brown bullhead (Ameiurus nebulosus), as the species most likely to spread and cause ecological harm in lakes. For 3431 lakes >1 ha throughout New Zealand, total IRI tended to be highest for lowland riverine and dune lakes most of which are already colonized by multiple invasive fish species.
3. The LV index indicated that lakes most at risk of loss of conservation value from invasive fish impacts were predominantly (i) in the northern half of the North Island where several uncommon lake types occur, and (ii) along the west coast of the South Island where conservation value is often greater, largely because of low catchment modification.
4. The IRI and LV indices can be used to assist with setting priorities for surveillance monitoring, advocacy, and response planning targeted at preventing the establishment of invasive fish in moderate‐to‐high value lakes most susceptible to ecological impacts. Both indices can be adapted to accommodate alternative impact and conservation scoring systems, providing a flexible tool for local‐ and national‐scale assessments of lake vulnerability to fish invasion impacts.

Copyright © 2016 John Wiley & Sons, Ltd.     

Motility of sea urchin Paracentrotus lividus spermatozoa in the post‐activation phase

The characterization of sperm motility patterns, particularly post‐activation changes, is the first step in setting up species‐specific protocols involving gamete management and embryo production, for both aquaculture and laboratory research purposes. This study is aimed at the characterization of the sperm motility pattern of the purple sea urchin Paracentrotus lividus. Semen samples were individually diluted in artificial sea water for sperm motility activation. They were then incubated at 18°C for up to 24 hr. Motility was evaluated on dilution, and 1 hr, 3 hr and 24 hr after activation, by computerized analyser. The semen fertilization capacity was also evaluated. Under our experimental conditions (dilution 1:1,000 in artificial sea water plus 0.05% BSA, 18°C, in the dark), P. lividus semen remained viable for up to 24 hr, as the total motile sperm and the fertilization percentages did not change significantly during the incubation time. In contrast, the mean curvilinear velocity and the subpopulation of rapid sperm (those having a curvilinear velocity > 100 µm/s) slightly but significantly decreased after 3 hr, thereafter remaining unchanged for up to 24 hr after activation. In conclusion, our results show that diluted P. lividus semen can be used for a longer period than that of most fish species, with no need for motility inhibition procedures, supporting its wider use in laboratory research. In addition, the development of artificial fertilization protocols for aquaculture production is simplified by long‐lasting sperm motility.     

Sorghum extrusion process combined with biofortified sweet potato contributed for high iron bioavailability in Wistar rats

This study aimed to evaluate the effect of sorghum and sweet potato on the bioavailability of iron, gene expression of proteins involved in iron metabolism and the plasma antioxidant capacity in animals fed with whole sorghum grains processed by dry heat or extrusion, combined or not with sweet potato flour with high content of carotenoids. Five experimental groups were tested (n = 7): dry heat sorghum flour (DS); extruded sorghum flour (ES); whole sorghum flour + sweet potato flour (DS + SP); extruded sorghum flour + sweet potato flour (ES + SP) and positive control (FS). The evaluations included: hemoglobin gain, hemoglobin regeneration efficiency, gene expression of divalente metal transporter 1 (DMT-1), duodenal citochroma B (DcytB), ferroportin, hephaestin, transferrin and ferritin and total plasma antioxidant capacity (TAC). The ES + SP group showed higher (p < 0.05) expression of DcytB, ferroportin and hephaestin when compared to the control group. The DS group showed high (p < 0.05) expression of DMT-1 and the ES showed high mRNA expression of transferrin and ferritin. The changes in the sorghum physicochemical properties from extrusion process reduced the iron and phytate content, and increased the gene expression of proteins involved in iron metabolism, improving iron bioavailability. The combination of sweet potato and sorghum flour (dry or extruded) improved the iron capture and total antioxidant capacity, probably due to the presence of β-carotene and antioxidant compounds.